Journal: Journal of Materials Chemistry. B
Article Title: Orthogonal conjugation of anchoring-dependent membrane active peptides for tuning of liposome permeability †
doi: 10.1039/d5tb00304k
Figure Lengend Snippet: (A) Total CF release after 1 h incubation with JR2KC (solid) and JR2KK-Az (dashed) on 40 μM MPB liposomes, with (black) or without (grey) cholesterol. Data were fitted to a Hill equation, N ≥ 8. (B) Hydrodynamic diameter of liposomes (40 μM) alone and after incubation with 1 μM JR2KC or JR2KK-Az for 1 h. ( p -value: * < 0.05, ** < 0.005 and *** < 0.0005). (C) Total CF release after 1 h incubation with JR2KC (solid) and JR2KK-Az (dashed) on 40 μM DBCO liposomes, with (blue) or without (cyan) cholesterol. Data were fitted to a Hill equation, N ≥ 8. (D) Total CF release from 40 μM MPB (grey) and MPB/Chol liposomes (black), and DBCO (cyan) and DBCO/Chol liposomes (blue), after 1 h incubation with JR2K. Data were fitted to a Hill equation, N ≥ 3. (E) Peptide concentration required to reach 20% CF release after 1 h incubation ([Pep] CF20% ) estimated from the fittings in (A) and (C). (F) Total CF release after 4 h incubation with JR2KC (solid) and JR2KK-Az (dashed) on liposomes (40 μM) with DBCO, with (blue) or without (cyan) cholesterol. Data were fitted to a Hill equation, N = 3. (G) Total CF release from DBCO-containing liposomes, with (blue) or without (cyan) cholesterol, obtained after 1 and 4 h, respectively, incubation with 4 μM JR2KC or JR2KK-Az. Data obtained from (C) and (F). (H) SPR sensorgrams showing the interaction of JR2KC and JR2KK-Az with MPB and DBCO liposomes on a Biacore L1 sensor chip. (I) Number of peptides per lipid after 10 min injection of JR2KC and JR2KK-Az over a MPB and DBCO liposome functionalized sensor chip.
Article Snippet: The SPR data were acquired using a Biacore T200 instrument and L1 sensor chips (Cytiva, Uppsala, Sweden).
Techniques: Incubation, Liposomes, Concentration Assay, Injection
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![(A) Total CF release after 1 h incubation with JR2KC (solid) and JR2KK-Az (dashed) on 40 μM MPB liposomes, with (black) or without (grey) cholesterol. Data were fitted to a Hill equation, N ≥ 8. (B) Hydrodynamic diameter of liposomes (40 μM) alone and after incubation with 1 μM JR2KC or JR2KK-Az for 1 h. ( p -value: * < 0.05, ** < 0.005 and *** < 0.0005). (C) Total CF release after 1 h incubation with JR2KC (solid) and JR2KK-Az (dashed) on 40 μM DBCO liposomes, with (blue) or without (cyan) cholesterol. Data were fitted to a Hill equation, N ≥ 8. (D) Total CF release from 40 μM MPB (grey) and MPB/Chol liposomes (black), and DBCO (cyan) and DBCO/Chol liposomes (blue), after 1 h incubation with JR2K. Data were fitted to a Hill equation, N ≥ 3. (E) Peptide concentration required to reach 20% CF release after 1 h incubation ([Pep] CF20% ) estimated from the fittings in (A) and (C). (F) Total CF release after 4 h incubation with JR2KC (solid) and JR2KK-Az (dashed) on liposomes (40 μM) with DBCO, with (blue) or without (cyan) cholesterol. Data were fitted to a Hill equation, N = 3. (G) Total CF release from DBCO-containing liposomes, with (blue) or without (cyan) cholesterol, obtained after 1 and 4 h, respectively, incubation with 4 μM JR2KC or JR2KK-Az. Data obtained from (C) and (F). <t>(H)</t> <t>SPR</t> sensorgrams showing the interaction of JR2KC and JR2KK-Az with MPB and DBCO liposomes on a Biacore <t>L1</t> sensor chip. (I) Number of peptides per lipid after 10 min injection of JR2KC and JR2KK-Az over a MPB and DBCO liposome functionalized sensor chip.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_1776/pmc12311776/pmc12311776__d5tb00304k-f1.jpg)